{"id":26485,"date":"2023-04-22T10:49:25","date_gmt":"2023-04-22T03:49:25","guid":{"rendered":"http:\/\/noithathoaphat.in\/ctv\/?p=26485"},"modified":"2023-05-02T10:01:50","modified_gmt":"2023-05-02T03:01:50","slug":"khai-niem-ve-biofilm-vi-khuan-noi-nha","status":"publish","type":"post","link":"http:\/\/noithathoaphat.in\/ctv\/index.php\/2023\/04\/22\/khai-niem-ve-biofilm-vi-khuan-noi-nha\/","title":{"rendered":"Kh\u00e1i ni\u1ec7m v\u1ec1 Biofilm vi khu\u1ea9n n\u1ed9i nha."},"content":{"rendered":"

Ch\u1ebf \u0111\u1ed9 sinh tr\u01b0\u1edfng c\u1ee7a biofilm l\u00e0 l\u1ed1i s\u1ed1ng \u01b0a th\u00edch c\u1ee7a vi khu\u1ea9n trong m\u00f4i tr\u01b0\u1eddng t\u1ef1 nhi\u00ean v\u00e0 nh\u00e2n t\u1ea1o. Khi vi khu\u1ea9n \u1edf pha l\u1ecfng, xu\u1ea5t hi\u1ec7n tr\u00ean nh\u1eefng b\u1ec1 m\u1eb7t c\u00f3 s\u1eb5n, v\u00ed d\u1ee5 nh\u01b0 c\u00e1c th\u00e0nh ng\u00e0 c\u1ee7a \u1ed1ng t\u1ee7y ch\u00e2n r\u0103ng, th\u00ec ch\u00fang c\u00f3 m\u1ed9t \u00e1i l\u1ef1c \u0111\u1ec3 b\u00e1m d\u00ednh v\u00e0 t\u1ea1o th\u00e0nh l\u1edbp biofilm. Biofilm c\u00f3 c\u1ea5u tr\u00fac kh\u00f4ng \u0111\u1ed3ng nh\u1ea5t, l\u00e0 n\u01a1i vi khu\u1ea9n c\u00f3 ki\u1ec3u h\u00ecnh v\u1edbi kh\u1ea3 n\u0103ng th\u00edch \u1ee9ng cao tr\u01b0\u1edbc \u00e1p l\u1ef1c, h\u00e0ng r\u00e0o mi\u1ec5n d\u1ecbch v\u00e0 thu\u1ed1c kh\u00e1ng sinh. C\u00f9ng t\u00ecm hi\u1ec3u.<\/strong><\/p>\n

1. Vi khu\u1ea9n t\u1ea1o th\u00e0nh biofilm nh\u01b0 th\u1ebf n\u00e0o?<\/h2>\n

Qu\u00e1 tr\u00ecnh t\u1ea1o th\u00e0nh biofilm c\u1ee7a vi khu\u1ea9n ph\u1ea3i tr\u1ea3i qua m\u1ed9t s\u1ed1 b\u01b0\u1edbc. Tr\u01b0\u1edbc ti\u00ean, m\u1ed9t t\u1ebf b\u00e0o t\u1ef1 do (planktonic) ti\u1ebfp x\u00fac v\u1edbi b\u1ec1 m\u1eb7t \u0111\u00e3 \u0111\u01b0\u1ee3c ph\u1ee7 s\u1eb5n protein (c\u1ea5u t\u1ea1o c\u1ee7a c\u00e1c th\u1ee5 th\u1ec3) c\u00f9ng v\u1edbi c\u00e1c ch\u1ea5t l\u1eafng \u0111\u1ecdng t\u1eeb m\u00f4i tr\u01b0\u1eddng. Sau \u0111\u00f3 t\u1ebf b\u00e0o vi khu\u1ea9n th\u00e0nh l\u1eadp s\u1ef1 li\u00ean k\u1ebft t\u1ea1m th\u1eddi v\u1edbi b\u1ec1 m\u1eb7t b\u1eb1ng chuy\u1ec3n \u0111\u1ed9ng Brownian, v\u1eadn chuy\u1ec3n ng\u01b0\u1ee3c d\u00f2ng, v\u00e0\/ho\u1eb7c bi\u1ec3u hi\u1ec7n c\u1ee7a c\u00e1c y\u1ebfu t\u1ed1 c\u1ea5u tr\u00fac nh\u01b0 tua v\u00e0 roi.<\/p>\n

Trong th\u1eddi gian ng\u1eafn, m\u1ed1i li\u00ean k\u1ebft b\u1ec1 m\u1eb7t t\u1ebf b\u00e0o n\u00e0y tr\u1edf n\u00ean \u1ed5n \u0111\u1ecbnh v\u00e0 b\u1eaft \u0111\u1ea7u s\u1ef1 h\u00ecnh th\u00e0nh c\u1ee7a c\u00e1c vi sinh v\u1eadt, ho\u1eb7c b\u1eb1ng s\u1ef1 k\u1ebft h\u1ee3p v\u1edbi c\u00e1c t\u1ebf b\u00e0o trong biofilm kh\u00e1c ho\u1eb7c b\u1eb1ng c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m sinh s\u1ea3n t\u00edch c\u1ef1c. C\u00e1c l\u1ef1c c\u01a1 h\u1ecdc \u1edf nh\u1eefng khu v\u1ef1c h\u1eb9p v\u00e0 kh\u00f4ng c\u00f3 h\u1ec7 th\u1ed1ng d\u00f2ng ch\u1ea3y nh\u01b0 l\u00e0 c\u1ea5u tr\u00fac ph\u1ee9c t\u1ea1p c\u1ee7a ch\u00f3p r\u0103ng s\u1ebd l\u00e0m thu\u1eadn l\u1ee3i cho s\u1ef1 h\u00ecnh th\u00e0nh c\u00e1c c\u1ee5m vi khu\u1ea9n\/biofilm n\u00e0y. T\u1ea7m quan tr\u1ecdng trong giai \u0111o\u1ea1n n\u00e0y l\u00e0 s\u1ef1 bi\u1ec3u hi\u1ec7n c\u1ee7a c\u00e1c polyme ngo\u1ea1i b\u00e0o (EPS) nhu polysaccharides, proteins, nucleic acids, and phospholipids, l\u00e0 nh\u1eefng th\u00e0nh ph\u1ea7n x\u00e2y d\u1ef1ng n\u00ean ch\u1ea5t n\u1ec1n, l\u00e0 khung x\u01b0\u01a1ng c\u1ee7a l\u1edbp biofilm. Cu\u1ed1i c\u00f9ng, s\u1ef1 tr\u01b0\u1edfng th\u00e0nh c\u1ee7a c\u00e1c c\u1ee5m vi khu\u1ea9n theo 3 chi\u1ec1u c\u1ea5u tr\u00fac s\u1ebd x\u1ea3y ra \u1edf ch\u1ea5t n\u1ec1n ngo\u1ea1i b\u00e0o. Th\u00f4ng th\u01b0\u1eddng, c\u00e1c vi khu\u1ea9n trong biofilm sau \u0111\u00f3 s\u1ebd t\u00e1ch ra kh\u1ecfi ch\u1ea5t n\u1ec1n. C\u00e1c b\u01b0\u1edbc th\u00e0nh l\u1eadp biofilm \u0111\u01b0\u1ee3c th\u1ec3 hi\u1ec7n trong h\u00ecnh d\u01b0\u1edbi \u0111\u00e2y.<\/p>\n

\"biofilm\"
S\u1ef1 ph\u00e1t tri\u1ec3n c\u1ee7a m\u1ed9t biofilm \u0111a ch\u1ee7ng lo\u00e0i theo th\u1eddi gian. (1) S\u1ef1 h\u00ecnh th\u00e0nh l\u1edbp m\u00e0ng \u0111\u1ea7u ti\u00ean tr\u00ean b\u1ec1 m\u1eb7t. (2) C\u00e1c t\u1ebf b\u00e0o vi khu\u1ea9n b\u00e1m d\u00ednh ban \u0111\u1ea7u. (3) S\u1ef1 g\u1eafn k\u1ebft kh\u00f4ng th\u1ec3 \u0111\u1ea3o ng\u01b0\u1ee3c, s\u1ef1 nh\u00e2n l\u00ean v\u00e0 s\u1ef1 h\u00ecnh th\u00e0nh c\u00e1c qu\u1ea7n th\u1ec3 nh\u1ecf. V\u00e0 (4) S\u1ef1 tr\u01b0\u1edfng th\u00e0nh c\u1ee7a biofilm, h\u00ecnh th\u00e0nh ch\u1ea5t n\u1ec1n.<\/figcaption><\/figure>\n

2. L\u1edbp m\u00e0ng vi khu\u1ea9n th\u00e0nh l\u1eadp \u1edf \u1ed1ng t\u1ee7y ch\u00e2n r\u0103ng<\/h2>\n

H\u1ec7 vi sinh v\u1eadt trong \u1ed1ng t\u1ee7y \u0111\u00e3 \u0111\u01b0\u1ee3c t\u00ecm th\u1ea5y khi ch\u00fang \u1ea9n n\u00e1u b\u1eb1ng c\u00e1ch b\u00e1m d\u00ednh v\u00e0o th\u00e0nh ng\u00e0 \u1edf t\u1ea5t c\u1ea3 c\u00e1c m\u1eb7t \u1ed1ng t\u1ee7y, ch\u1eb3ng h\u1ea1n nh\u01b0 th\u00e0nh trong c\u1ee7a ch\u00f3p ho\u1eb7c c\u00e1c \u1ed1ng t\u1ee7y ph\u1ee5. V\u00e0o n\u0103m 2004, Svensater and Bergenholtz \u0111\u1ec1 xu\u1ea5t m\u1ed9t gi\u1ea3 thuy\u1ebft v\u1ec1 s\u1ef1 h\u00ecnh th\u00e0nh biofilm \u1edf \u1ed1ng t\u1ee7y ch\u00e2n r\u0103ng. Qu\u00e1 tr\u00ecnh th\u00e0nh l\u1eadp biofilm \u1edf \u1ed1ng t\u1ee7y \u0111\u01b0\u1ee3c b\u1eaft \u0111\u1ea7u tr\u1ef1c ti\u1ebfp ngay sau cu\u1ed9c x\u00e2m nh\u1eadp \u0111\u1ea7u ti\u00ean c\u1ee7a vi khu\u1ea9n v\u00e0o bu\u1ed3ng t\u1ee7y v\u00e0 l\u00e0m ph\u00e2n h\u1ee7y qu\u00e1 tr\u00ecnh vi\u00eam. V\u1ecb tr\u00ed ban \u0111\u1ea7u c\u1ee7a t\u1ed5n th\u01b0\u01a1ng vi\u00eam s\u1ebd di chuy\u1ec3n d\u1ea7n v\u1ec1 ph\u00eda ch\u00f3p, cung c\u1ea5p d\u00f2ng d\u1ecbch chuy\u1ec3n cho c\u00e1c vi sinh v\u1eadt x\u00e2m nh\u1eadp \u0111\u1ec3 ch\u00fang c\u00f3 th\u1ec3 nh\u00e2n l\u00ean v\u00e0 ti\u1ebfp t\u1ee5c g\u1eafn v\u00e0o c\u00e1c th\u00e0nh \u1ed1ng t\u1ee7y. Gi\u1ea3 thi\u1ebft n\u00e0y \u0111\u00e3 \u0111\u01b0\u1ee3c x\u00e1c minh b\u1eb1ng nh\u1eefng quan s\u00e1t tr\u00ean k\u00ednh hi\u1ec3n vi quang h\u1ecdc, \u0111\u00e3 th\u1ea5y vi khu\u1ea9n t\u00e1ch ra t\u1eeb m\u1eb7t trong c\u1ee7a \u1ed1ng t\u1ee7y. \u0110i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 gi\u00fap gi\u1ea3i th\u00edch l\u00e0m th\u1ebf n\u00e0o m\u00e0 t\u1ed5n th\u01b0\u01a1ng vi\u00eam tr\u1edf th\u00e0nh 1 ngu\u1ed3n dinh d\u01b0\u1ee1ng cho vi khu\u1ea9n trong biofilm ph\u00e2n t\u00e1ch v\u00e0 \u1ea9n n\u00e1u t\u1ea1i khu v\u1ef1c ch\u00f3p \u1ed1ng t\u1ee7y v\u00e0 nh\u1eefng v\u00f9ng xa h\u01a1n.<\/p>\n

3. T\u1ea1i sao vi khu\u1ea9n l\u1ea1i th\u00e0nh l\u1eadp l\u1edbp m\u00e0ng n\u00e0y?<\/h2>\n

Sinh l\u00fd h\u1ecdc c\u1ee7a vi khu\u1ea9n trong c\u1ed9ng \u0111\u1ed3ng planktonic (vi sinh v\u1eadt tr\u00f4i n\u1ed5i t\u1ef1 do) r\u1ea5t kh\u00e1c bi\u1ec7t so v\u1edbi nh\u1eefng vi sinh v\u1eadt kh\u00e1c c\u00f9ng ph\u00e1t tri\u1ec3n tr\u00ean b\u1ec1 m\u1eb7t biofilm. Vi khu\u1ea9n planktonic th\u01b0\u1eddng nh\u1ea1y c\u1ea3m h\u01a1n v\u1edbi c\u00e1c t\u00e1c nh\u00e2n kh\u00e1ng khu\u1ea9n do ch\u00fang ti\u1ebfp x\u00fac v\u1edbi m\u1ed9t l\u01b0\u1ee3ng l\u1edbn c\u00e1c t\u00e1c nh\u00e2n n\u00e0y. Nh\u1eefng ki\u1ebfn th\u1ee9c hi\u1ec7n nay cho th\u1ea5y s\u1ef1 h\u00ecnh th\u00e0nh biofilm \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh \u0111\u1ec3 \u0111\u00e1p \u1ee9ng v\u1edbi \u0111i\u1ec1u ki\u1ec7n m\u00f4i tr\u01b0\u1eddng r\u1ea5t kh\u00e1c nhau gi\u1eefa c\u00e1c lo\u00e0i kh\u00e1c nhau. Sau khi m\u1ed9t t\u1ebf b\u00e0o planktonic \u0111\u01b0\u1ee3c nh\u1eadn di\u1ec7n l\u00e0 m\u1ed9t \u201ct\u00edn hi\u1ec7u b\u1ec1 m\u1eb7t\u201d, th\u00ec 1 s\u1ed1 c\u01a1 ch\u1ebf \u0111i\u1ec1u ch\u1ec9nh s\u1ebd x\u1ea3y ra trong t\u1ebf b\u00e0o, l\u00e0m chuy\u1ec3n \u0111\u1ed5i ch\u1ebf \u0111\u1ed9 t\u1eeb tr\u00f4i t\u1ef1 do th\u00e0nh biofilm. C\u00e1c c\u01a1 ch\u1ebf \u0111i\u1ec1u ch\u1ec9nh n\u00e0y, hay c\u00f2n g\u1ecdi l\u00e0 c\u1ea3m nh\u1eadn b\u1ec1 m\u1eb7t, trong nhi\u1ec1u tr\u01b0\u1eddng h\u1ee3p c\u00f3 li\u00ean quan \u0111\u1ebfn c\u00e1c receptor \u0111\u1eb7c bi\u1ec7t ngo\u00e0i l\u1edbp m\u00e0ng t\u1ebf b\u00e0o v\u00e0 nh\u1eefng t\u00edn hi\u1ec7u ch\u1eb3ng h\u1ea1n nh\u01b0 roi c\u1ee7a vi khu\u1ea9n. Vai tr\u00f2 c\u1ea3m bi\u1ebfn tr\u01b0\u1edbc ti\u00ean n\u00e0y c\u1ee7a roi vi khu\u1ea9n r\u1ea5t quan tr\u1ecdng trong giai \u0111o\u1ea1n kh\u1edfi ph\u00e1t h\u00ecnh th\u00e0nh biofilm, l\u00e0 y\u1ebfu t\u1ed1 t\u00edn hi\u1ec7u g\u00e2y ra 1 lo\u1ea1t c\u00e1c ho\u1ea1t \u0111\u1ed9ng n\u1ed9i b\u00e0o d\u1eabn \u0111\u1ebfn s\u1ef1 h\u00ecnh th\u00e0nh c\u00e1c ki\u1ec3u h\u00ecnh biofilm. Nh\u1eefng y\u00eau c\u1ea7u v\u1ec1 ki\u1ec3u h\u00ecnh biofilm n\u00e0y s\u1ebd l\u00e0m cho vi khu\u1ea9n \u0111\u01b0\u1ee3c b\u1ea3o v\u1ec7 kh\u1ecfi c\u00e1c t\u00e1c nh\u00e2n ch\u1ed1ng l\u1ea1i ch\u00fang, ch\u1ee7 y\u1ebfu nh\u1edd v\u00e0o s\u1ef1 h\u00ecnh th\u00e0nh qu\u1ea7n th\u1ec3 kh\u00f4ng \u0111\u1ed3ng nh\u1ea5t trong biofilm, v\u1edbi s\u1ef1 kh\u00e1c bi\u1ec7t v\u1ec1 t\u1ed1c \u0111\u1ed9 t\u0103ng tr\u01b0\u1edfng v\u00e0 bi\u1ec3u hi\u1ec7n c\u1ee7a gen. T\u00ednh ch\u1ea5t kh\u00f4ng \u0111\u1ed3ng nh\u1ea5t c\u1ee7a biofilm, c\u00f9ng v\u1edbi s\u1ef1 ph\u1ed1i h\u1ee3p v\u00e0 r\u00e0ng bu\u1ed9c c\u00f3 ch\u1ecdc l\u1ecdc c\u1ee7a c\u00e1c y\u1ebfu t\u1ed1 kh\u00e1ng khu\u1ea9n, l\u00e0m cho biofilm tr\u1edf th\u00e0nh 1 m\u1ee5c ti\u00eau \u0111i\u1ec1u tr\u1ecb kh\u00f3 kh\u0103n.<\/p>\n

4. S\u1ef1 \u0111\u1ec1 kh\u00e1ng c\u1ee7a biofilm v\u1edbi c\u00e1c y\u1ebfu t\u1ed1 kh\u00e1ng khu\u1ea9n<\/h2>\n

Th\u00f4ng th\u01b0\u1eddng, hi\u1ec7u qu\u1ea3 c\u1ee7a c\u00e1c y\u1ebfu t\u1ed1 kh\u00e1ng khu\u1ea9n trong \u0111i\u1ec1u tr\u1ecb n\u1ed9i nha \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 d\u1ef1a v\u00e0o ho\u1ea1t t\u00ednh c\u1ee7a ch\u00fang \u0111\u1ed1i v\u1edbi vi sinh v\u1eadt \u0111\u01b0\u1ee3c nu\u00f4i c\u1ea5y trong m\u00f4i tr\u01b0\u1eddng in vitro. Tuy nhi\u00ean, h\u1ec7 th\u1ed1ng n\u00e0y s\u1eed d\u1ee5ng t\u1ebf b\u00e0o planktonic kh\u00f4ng th\u1ec3 hi\u1ec7n \u0111\u00fang v\u1edbi tr\u1ea1ng th\u00e1i nh\u01b0 in vivo v\u00ec c\u00e1c vi sinh v\u1eadt \u0111\u01b0\u1ee3c \u0111\u1ecbnh h\u01b0\u1edbng h\u01a1n l\u00e0 \u0111\u01b0\u1ee3c tr\u00f4i n\u1ed5i t\u1ef1 do trong c\u1ea5u tr\u00fac c\u1ee7a biofilm \u1edf th\u00e0nh \u1ed1ng t\u1ee7y. C\u1ed9ng \u0111\u1ed3ng vi sinh v\u1eadt trong biofilm n\u00e0y c\u00f3 s\u1ee9c \u0111\u1ec1 kh\u00e1ng \u0111\u00e1ng k\u1ec3 v\u00e0 kh\u00f3 lo\u1ea1i tr\u1eeb tri\u1ec7t \u0111\u1ec3 b\u1eb1ng c\u00e1c ch\u1ea5t kh\u00e1ng khu\u1ea9n.<\/p>\n

S\u1ef1 gia t\u0103ng kh\u1ea3 n\u0103ng \u0111\u1ec1 kh\u00e1ng c\u1ee7a c\u00e1c lo\u1ea1i t\u1ebf b\u00e0o vi khu\u1ea9n trong biofilm so v\u1edbi c\u00e1c t\u1ebf b\u00e0o planktonic \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh b\u1edfi m\u1ed9t s\u1ed1 c\u01a1 ch\u1ebf bao g\u1ed3m s\u1ef1 th\u00e2m nh\u1eadp ch\u1eadm c\u1ee7a y\u1ebfu t\u1ed1 kh\u00e1ng khu\u1ea9n v\u00e0o biofilm, s\u1ef1 thay \u0111\u1ed5i m\u00f4i tr\u01b0\u1eddng vi sinh h\u00f3a trong biofilm (t\u1ea1o th\u00e0nh c\u00e1c v\u00f9ng ch\u1eadm t\u0103ng tr\u01b0\u1edfng ho\u1eb7c kh\u00f4ng t\u0103ng tr\u01b0\u1edfng), th\u00edch nghi v\u1edbi c\u00e1c ph\u1ea3n \u1ee9ng l\u1ef1c v\u00e0 s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a m\u1ed9t s\u1ed1 l\u01b0\u1ee3ng l\u1edbn c\u00e1c t\u1ebf b\u00e0o si\u00eau kh\u00e1ng. Nh\u1eefng t\u1ebf b\u00e0o si\u00eau kh\u00e1ng n\u00e0y c\u00f3 th\u1ec3 ch\u1ecbu \u0111\u01b0\u1ee3c c\u00e1c t\u00e1c nh\u00e2n kh\u00e1ng khu\u1ea9n (t\u1ee9c l\u00e0 ch\u00fang s\u1ebd kh\u00f4ng b\u1ecb ti\u00eau di\u1ec7t), v\u00e0 c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c xem l\u00e0 nh\u1eefng th\u1ef1c th\u1ec3 s\u1ed1ng \u0111\u1eb7c bi\u1ec7t.<\/p>\n

Nghi\u00ean c\u1ee9u v\u1ec1 s\u1ee9c \u0111\u1ec1 kh\u00e1ng c\u1ee7a vi khu\u1ea9n trong biofilm \u0111\u1ed1i v\u1edbi c\u00e1c ch\u1ea5t kh\u00e1ng khu\u1ea9n \u0111\u00f2i h\u1ecfi m\u00f4 h\u00ecnh in vitro ph\u1ea3i g\u1ea7n gi\u1ed1ng v\u1edbi \u0111i\u1ec1u ki\u1ec7n l\u00e2m s\u00e0ng. M\u1eb7c d\u00f9 c\u00e1c th\u00ed nghi\u1ec7m v\u1ec1 t\u00e1c nh\u00e2n kh\u00e1ng khu\u1ea9n ch\u1ed1ng l\u1ea1i vi khu\u1ea9n trong biofilm ch\u01b0a \u0111\u01b0\u1ee3c ti\u00eau chu\u1ea9n h\u00f3a, nh\u01b0ng nhi\u1ec1u nghi\u00ean c\u1ee9u \u0111\u00e3 s\u1eed d\u1ee5ng m\u00f4 h\u00ecnh lo\u1ea1i b\u1ecf c\u00e1c t\u1ebf b\u00e0o biofilm b\u1eb1ng c\u01a1 h\u1ecdc sau khi nu\u00f4i c\u1ea5y t\u1ebf b\u00e0o theo h\u00ecnh th\u1ee9c truy\u1ec1n th\u1ed1ng \u0111\u1ec3 nghi\u00ean c\u1ee9u (Colony Forming Units – CFU). C\u00e1c ph\u01b0\u01a1ng ph\u00e1p kh\u00e1c bao g\u1ed3m s\u1eed d\u1ee5ng k\u00ednh hi\u1ec3n vi k\u1ebft h\u1ee3p v\u1edbi kh\u1ea3 n\u0103ng b\u1eaft m\u00e0u thu\u1ed1c nhu\u1ed9m hu\u1ef3nh quang \u0111\u00ea quan s\u00e1t t\u1ea1i ch\u1ed7 nh\u1eefng hi\u1ec7u \u1ee9ng kh\u00e1ng khu\u1ea9n tr\u00ean biofilm. Trong m\u1ed9t b\u00e0i b\u00e1o c\u00e1o g\u1ea7n \u0111\u00e2y, m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch biofilm \u0111\u00e3 \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y bao g\u1ed3m vi\u1ec7c \u0111\u00e1nh gi\u00e1 kh\u1ea3 n\u0103ng t\u1ed3n t\u1ea1i c\u1ee7a m\u00e0ng t\u1ebf b\u00e0o v\u00e0 c\u1ea5u tr\u00fac c\u1ee7a biofilm \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 t\u1ea1i ch\u1ed7 hi\u1ec7u qu\u1ea3 c\u1ee7a c\u00e1c ch\u1ea5t kh\u00e1ng khu\u1ea9n l\u00ean vi khu\u1ea9n tr\u00ean biofilm. Ng\u01b0\u1eddi ta x\u00e1c \u0111\u1ecbnh r\u1eb1ng sau khi ki\u1ec3m so\u00e1t c\u00e1c bi\u1ebfn s\u1ed1 nh\u01b0 \u0111i\u1ec1u ki\u1ec7n ch\u1ea5t n\u1ec1n th\u00ec s\u1ef1 thay \u0111\u1ed5i c\u1ea5u tr\u00fac c\u1ee7a biofilm v\u00e0 s\u1ef1 ph\u00e2n b\u1ed1 c\u00e1c t\u1ebf b\u00e0o v\u1edbi ho\u1ea1t \u0111\u1ed9ng chuy\u1ec3n h\u00f3a m\u1ea1nh m\u1ebd c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c theo d\u00f5i sau khi s\u1eed d\u1ee5ng c\u00e1c t\u00e1c nh\u00e2n kh\u00e1ng khu\u1ea9n tr\u00ean l\u00e2m s\u00e0ng.<\/p>\n

NaOCl (1%) \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn t\u00ednh to\u00e0n v\u1eb9n c\u1ee7a m\u00e0ng t\u1ebf b\u00e0o c\u1ee7a t\u1ea5t c\u1ea3 c\u00e1c lo\u1ea1i vi sinh v\u1eadt \u0111\u01b0\u1ee3c th\u1eed nghi\u1ec7m v\u00e0 lo\u1ea1i b\u1ecf h\u1ea7u h\u1ebft c\u00e1c t\u1ebf b\u00e0o biofilm. S\u1ef1 ti\u1ebfp x\u00fac v\u1edbi EDTA (50 mmol\/l) c\u0169ng \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn t\u00ednh to\u00e0n v\u1eb9n c\u1ee7a l\u1edbp m\u00e0ng \u1edf t\u1ea5t c\u1ea3 c\u00e1c ch\u1ee7ng vi sinh v\u1eadt nh\u01b0ng ch\u1ec9 lo\u1ea1i b\u1ecf \u0111\u01b0\u1ee3c m\u1ed9t l\u01b0\u1ee3ng \u00edt vi khu\u1ea9n trong biofilm nh\u01b0 E. faecalis, L. paracasei, v\u00e0 S. anginosus. Chlorhexidine (2.5 %) c\u00f3 \u00edt \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn t\u00ednh to\u00e0n v\u1eb9n c\u1ee7a m\u00e0ng t\u1ebf b\u00e0o \u1edf E. faecalis v\u00e0 ch\u1ec9 lo\u1ea1i b\u1ecf \u0111\u01b0\u1ee3c 50% c\u00e1c t\u1ebf b\u00e0o biofilm c\u1ee7a ch\u00fang.<\/p>\n

C\u1ea7n l\u01b0u \u00fd r\u1eb1ng t\u00e1c d\u1ee5ng kh\u00e1ng khu\u1ea9n c\u00f2n ph\u1ee5 thu\u1ed9c v\u00e0o b\u1ec1 m\u1eb7t ch\u1ee9a vi khu\u1ea9n, v\u00e0 h\u1ea7u h\u1ebft c\u00e1c ch\u1ee7ng vi sinh v\u1eadt \u0111\u1ec1u th\u1ec3 hi\u1ec7n kh\u1ea3 n\u0103ng kh\u00e1ng l\u1ea1i c\u00e1c y\u1ebfu t\u1ed1 kh\u00e1ng khu\u1ea9n ng\u00e0y m\u1ed9t m\u1ea1nh h\u01a1n tr\u00ean nh\u1eefng b\u1ec1 m\u1eb7t c\u00f3 ph\u1ee7 collagen. Nh\u1eefng ph\u00e1t hi\u1ec7n n\u00e0y cung c\u1ea5p th\u00eam nhi\u1ec1u b\u1eb1ng ch\u1ee9ng cho th\u1ea5y vi khu\u1ea9n trong c\u00e1c c\u1ed9ng \u0111\u1ed3ng \u0111\u1ed3ng nh\u1ea5t \u1edf l\u1edbp biofilm b\u00e1m d\u00ednh tr\u00ean b\u1ec1 m\u1eb7t c\u00f3 kh\u1ea3 n\u0103ng \u0111\u1ec1 kh\u00e1ng r\u1ea5t thay \u0111\u1ed5i \u0111\u1ed1i v\u1edbi c\u00e1c ch\u1ea5t kh\u00e1ng khu\u1ea9n, m\u00e0 \u0111i\u1ec1u n\u00e0y th\u01b0\u1eddng \u0111\u00f2i h\u1ecfi m\u00f4 h\u00ecnh biofilm \u0111a t\u1ea1p c\u00f3 kh\u1ea3 n\u0103ng t\u00e1i sinh.<\/p>\n

Ngu\u1ed3n: Clinical Guide for Optimal Treatment Outcome – Springer International Publishing Switzerland 2017<\/span><\/em><\/p>\n","protected":false},"excerpt":{"rendered":"

Ch\u1ebf \u0111\u1ed9 sinh tr\u01b0\u1edfng c\u1ee7a biofilm l\u00e0 l\u1ed1i s\u1ed1ng \u01b0a th\u00edch c\u1ee7a vi khu\u1ea9n trong m\u00f4i tr\u01b0\u1eddng t\u1ef1 nhi\u00ean v\u00e0 nh\u00e2n t\u1ea1o. Khi vi khu\u1ea9n \u1edf pha l\u1ecfng, xu\u1ea5t hi\u1ec7n tr\u00ean nh\u1eefng b\u1ec1 m\u1eb7t c\u00f3 s\u1eb5n, v\u00ed d\u1ee5 nh\u01b0 c\u00e1c th\u00e0nh ng\u00e0 c\u1ee7a \u1ed1ng t\u1ee7y ch\u00e2n r\u0103ng, th\u00ec ch\u00fang c\u00f3 m\u1ed9t \u00e1i l\u1ef1c \u0111\u1ec3 b\u00e1m […]<\/p>\n","protected":false},"author":21,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[28,33],"tags":[],"yoast_head":"\nKh\u00e1i ni\u1ec7m v\u1ec1 Biofilm vi khu\u1ea9n n\u1ed9i nha. - Vinmec<\/title>\n<meta name=\"description\" content=\"Biofilm c\u1ea5u tr\u00fac kh\u00f4ng \u0111\u1ed3ng nh\u1ea5t, l\u00e0 n\u01a1i vi khu\u1ea9n c\u00f3 kh\u1ea3 n\u0103ng th\u00edch \u1ee9ng cao tr\u01b0\u1edbc \u00e1p l\u1ef1c, mi\u1ec5n d\u1ecbch v\u00e0 thu\u1ed1c kh\u00e1ng sinh. 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