{"id":2249,"date":"2023-04-11T22:08:25","date_gmt":"2023-04-11T15:08:25","guid":{"rendered":"http:\/\/noithathoaphat.in\/ctv\/?p=2249"},"modified":"2023-04-14T12:45:19","modified_gmt":"2023-04-14T05:45:19","slug":"phan-biet-northern-southern-eastern-va-western-blot","status":"publish","type":"post","link":"http:\/\/noithathoaphat.in\/ctv\/index.php\/2023\/04\/11\/phan-biet-northern-southern-eastern-va-western-blot\/","title":{"rendered":"Ph\u00e2n bi\u1ec7t Northern, Southern, Eastern v\u00e0 Western blot"},"content":{"rendered":"<p><strong>Northern, Southern, Eastern v\u00e0 Western blot l\u00e0 4 k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch ph\u00e2n t\u1eed v\u1edbi nhi\u1ec1u \u1ee9ng d\u1ee5ng nh\u01b0ng d\u1ec5 g\u00e2y nh\u1ea7m l\u1eabn v\u00e0 kh\u00f3 ph\u00e2n bi\u1ec7t. B\u00e0i vi\u1ebft s\u1ebd cung c\u1ea5p nh\u1eefng \u0111i\u1ec3m ch\u00ednh y\u1ebfu v\u00e0 ph\u00e2n bi\u1ec7t 4 ph\u01b0\u01a1ng ph\u00e1p n\u00e0y.<\/strong><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"size-medium wp-image-2251 aligncenter\" src=\"http:\/\/noithathoaphat.in\/ctv\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-08-at-21.14.20-300x294.png\" alt=\"\" width=\"300\" height=\"294\" srcset=\"http:\/\/noithathoaphat.in\/ctv\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-08-at-21.14.20-300x294.png 300w, http:\/\/noithathoaphat.in\/ctv\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-08-at-21.14.20.png 736w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<h4>1. K\u1ef9 thu\u1eadt Nothern blot<\/h4>\n<p>K\u1ef9 thu\u1eadt Northern blot l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh m\u1ee9c \u0111\u1ed9 bi\u1ec3u hi\u1ec7n gen v\u00e0 kh\u1ea3o s\u00e1t c\u00e1c s\u1ea3n ph\u1ea9m c\u1ee7a ch\u00fang. K\u1ef9 thu\u1eadt n\u00e0y s\u1eed d\u1ee5ng nguy\u00ean l\u00fd c\u1ee7a \u0111i\u1ec7n di \u0111\u1ec3 ph\u00e2n t\u00e1ch c\u00e1c RNA theo k\u00edch th\u01b0\u1edbc v\u00e0 sau \u0111\u00f3 s\u1eed d\u1ee5ng c\u00e1c kh\u00e1ng th\u1ec3 \u0111\u1eb7c hi\u1ec7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n.<\/p>\n<p>Qu\u00e1 tr\u00ecnh Northern blot bao g\u1ed3m c\u00e1c b\u01b0\u1edbc nh\u01b0 sau: \u0111\u1ea7u ti\u00ean, RNA \u0111\u01b0\u1ee3c chi\u1ebft xu\u1ea5t t\u1eeb m\u1eabu v\u00e0 sau \u0111\u00f3 \u0111\u01b0\u1ee3c t\u00e1ch ra b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p \u0111i\u1ec7n di tr\u00ean gel agarose ho\u1eb7c polyacrylamide \u0111\u1ec3 ph\u00e2n t\u00e1ch c\u00e1c RNA theo k\u00edch th\u01b0\u1edbc. Sau \u0111\u00f3, c\u00e1c RNA \u0111\u01b0\u1ee3c chuy\u1ec3n t\u1eeb gel l\u00ean m\u00e0ng nitrocellulose ho\u1eb7c nylon v\u00e0 \u0111\u01b0\u1ee3c l\u00e0m kh\u00f4. M\u00e0ng \u0111\u01b0\u1ee3c x\u1eed l\u00fd b\u1eb1ng m\u1ed9t kh\u00e1ng th\u1ec3 \u0111\u1eb7c hi\u1ec7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a RNA c\u1ee5 th\u1ec3. Th\u00f4ng th\u01b0\u1eddng, kh\u00e1ng th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c g\u1eafn v\u1edbi m\u1ed9t ph\u1ea7n t\u1eed s\u1eafc t\u1ed1 ho\u1eb7c enzyme \u0111\u1ec3 t\u1ea1o ra t\u00edn hi\u1ec7u d\u01b0\u01a1ng t\u00ednh. Cu\u1ed1i c\u00f9ng, m\u00e0ng \u0111\u01b0\u1ee3c qu\u00e9t \u0111\u1ec3 ph\u00e1t hi\u1ec7n t\u00edn hi\u1ec7u v\u00e0 x\u00e1c \u0111\u1ecbnh m\u1ee9c \u0111\u1ed9 bi\u1ec3u hi\u1ec7n c\u1ee7a RNA c\u1ea7n x\u00e1c \u0111\u1ecbnh.<\/p>\n<p>K\u1ef9 thu\u1eadt Northern blot l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch quan tr\u1ecdng \u0111\u1ec3 nghi\u00ean c\u1ee9u bi\u1ec3u hi\u1ec7n gen trong c\u00e1c \u0111i\u1ec1u ki\u1ec7n kh\u00e1c nhau. Tuy nhi\u00ean, k\u1ef9 thu\u1eadt n\u00e0y \u0111\u00f2i h\u1ecfi nhi\u1ec1u th\u1eddi gian v\u00e0 c\u00f4ng s\u1ee9c \u0111\u1ec3 th\u1ef1c hi\u1ec7n v\u00e0 c\u1ea7n ph\u1ea3i \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n c\u1ea9n th\u1eadn v\u00e0 ch\u00ednh x\u00e1c \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o t\u00ednh ch\u00ednh x\u00e1c v\u00e0 \u0111\u1ed9 nh\u1ea1y c\u1ee7a k\u1ebft qu\u1ea3. Ngo\u00e0i ra, k\u1ef9 thu\u1eadt Northern blot c\u0169ng \u0111\u00e3 b\u1ecb thay th\u1ebf b\u1edfi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch gen m\u1edbi nh\u01b0 RT-PCR v\u00e0 RNA-seq.<\/p>\n<h4>2. K\u1ef9 thu\u1eadt Southern blot<\/h4>\n<p>K\u1ef9 thu\u1eadt Southern blot l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e2n t\u00edch v\u00e0 x\u00e1c \u0111\u1ecbnh s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a m\u1ed9t DNA c\u1ee5 th\u1ec3 trong m\u1ed9t m\u1eabu. K\u1ef9 thu\u1eadt n\u00e0y s\u1eed d\u1ee5ng nguy\u00ean l\u00fd c\u1ee7a \u0111i\u1ec7n di \u0111\u1ec3 t\u00e1ch c\u00e1c DNA trong m\u1eabu theo k\u00edch th\u01b0\u1edbc v\u00e0 sau \u0111\u00f3 s\u1eed d\u1ee5ng c\u00e1c kh\u00e1ng th\u1ec3 \u0111\u1eb7c hi\u1ec7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n DNA c\u1ee5 th\u1ec3.<\/p>\n<p>Qu\u00e1 tr\u00ecnh Southern blot bao g\u1ed3m c\u00e1c b\u01b0\u1edbc nh\u01b0 sau: \u0111\u1ea7u ti\u00ean, DNA \u0111\u01b0\u1ee3c c\u1eaft th\u00e0nh c\u00e1c \u0111o\u1ea1n b\u1eb1ng c\u00e1c enzyme c\u1eaft DNA c\u1ee5 th\u1ec3 v\u00e0 sau \u0111\u00f3 \u0111\u01b0\u1ee3c t\u00e1ch ra b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p \u0111i\u1ec7n di tr\u00ean gel agarose \u0111\u1ec3 ph\u00e2n t\u00e1ch c\u00e1c DNA theo k\u00edch th\u01b0\u1edbc. Sau \u0111\u00f3, c\u00e1c DNA \u0111\u01b0\u1ee3c chuy\u1ec3n t\u1eeb gel l\u00ean m\u00e0ng nitrocellulose ho\u1eb7c nylon v\u00e0 \u0111\u01b0\u1ee3c l\u00e0m kh\u00f4. M\u00e0ng \u0111\u01b0\u1ee3c x\u1eed l\u00fd b\u1eb1ng m\u1ed9t kh\u00e1ng th\u1ec3 \u0111\u1eb7c hi\u1ec7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a DNA c\u1ee5 th\u1ec3. Th\u00f4ng th\u01b0\u1eddng, kh\u00e1ng th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c g\u1eafn v\u1edbi m\u1ed9t ph\u1ea7n t\u1eed s\u1eafc t\u1ed1 ho\u1eb7c enzyme \u0111\u1ec3 t\u1ea1o ra t\u00edn hi\u1ec7u d\u01b0\u01a1ng t\u00ednh. Cu\u1ed1i c\u00f9ng, m\u00e0ng \u0111\u01b0\u1ee3c qu\u00e9t \u0111\u1ec3 ph\u00e1t hi\u1ec7n t\u00edn hi\u1ec7u v\u00e0 x\u00e1c \u0111\u1ecbnh s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a DNA c\u1ea7n x\u00e1c \u0111\u1ecbnh.<\/p>\n<p>K\u1ef9 thu\u1eadt Southern blot l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch quan tr\u1ecdng \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh s\u1ef1 hi\u1ec7n di\u1ec7n v\u00e0 t\u01b0\u01a1ng \u1ee9ng c\u1ee7a DNA c\u1ee5 th\u1ec3 trong m\u1eabu. N\u00f3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i trong nghi\u00ean c\u1ee9u sinh h\u1ecdc, y h\u1ecdc, v\u00e0 c\u00e1c l\u0129nh v\u1ef1c li\u00ean quan \u0111\u1ebfn DNA \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh t\u1ed5ng s\u1ed1 b\u1ea3n sao c\u1ee7a m\u1ed9t gen, t\u00ecm ki\u1ebfm \u0111\u1ed9t bi\u1ebfn gen, v\u00e0 kh\u1ea3o s\u00e1t c\u00e1c v\u00f9ng gen quan tr\u1ecdng. Tuy nhi\u00ean, qu\u00e1 tr\u00ecnh Southern blot c\u1ea7n \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n c\u1ea9n th\u1eadn v\u00e0 ch\u00ednh x\u00e1c \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o t\u00ednh ch\u00ednh x\u00e1c v\u00e0 \u0111\u1ed9 nh\u1ea1y c\u1ee7a k\u1ebft qu\u1ea3. Ngo\u00e0i ra, k\u1ef9 thu\u1eadt Southern blot c\u0169ng \u0111\u00e3 b\u1ecb thay th\u1ebf b\u1edfi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch gen m\u1edbi nh\u01b0 PCR v\u00e0 DNA sequencing.<\/p>\n<p><strong>3. K\u1ef9 thu\u1eadt Western blot\u00a0<\/strong><\/p>\n<p>K\u1ef9 thu\u1eadt Western blot l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p ph\u1ed5 bi\u1ebfn \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e2n t\u00edch v\u00e0 x\u00e1c \u0111\u1ecbnh s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a m\u1ed9t protein c\u1ee5 th\u1ec3 trong m\u1eabu. K\u1ef9 thu\u1eadt n\u00e0y s\u1eed d\u1ee5ng nguy\u00ean l\u00fd c\u1ee7a \u0111i\u1ec7n di \u0111\u1ec3 t\u00e1ch c\u00e1c protein trong m\u1eabu theo k\u00edch th\u01b0\u1edbc v\u00e0 sau \u0111\u00f3 s\u1eed d\u1ee5ng kh\u00e1ng th\u1ec3 \u0111\u1eb7c hi\u1ec7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n protein c\u1ea7n x\u00e1c \u0111\u1ecbnh.<\/p>\n<p>Qu\u00e1 tr\u00ecnh Western blot bao g\u1ed3m c\u00e1c b\u01b0\u1edbc nh\u01b0 sau: \u0111\u1ea7u ti\u00ean, m\u1eabu protein \u0111\u01b0\u1ee3c t\u00e1ch ra b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p \u0111i\u1ec7n di tr\u00ean gel SDS-PAGE \u0111\u1ec3 ph\u00e2n t\u00e1ch c\u00e1c protein theo tr\u1ecdng l\u01b0\u1ee3ng ph\u00e2n t\u1eed. Sau \u0111\u00f3, c\u00e1c protein \u0111\u01b0\u1ee3c chuy\u1ec3n t\u1eeb gel l\u00ean m\u00e0ng nitrocellulose ho\u1eb7c PVDF v\u00e0 \u0111\u01b0\u1ee3c l\u00e0m kh\u00f4. M\u00e0ng \u0111\u01b0\u1ee3c x\u1eed l\u00fd b\u1eb1ng m\u1ed9t kh\u00e1ng th\u1ec3 \u0111\u1eb7c hi\u1ec7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a protein c\u1ee5 th\u1ec3. Th\u00f4ng th\u01b0\u1eddng, kh\u00e1ng th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c g\u1eafn v\u1edbi m\u1ed9t ph\u1ea7n t\u1eed s\u1eafc t\u1ed1 ho\u1eb7c enzyme \u0111\u1ec3 t\u1ea1o ra t\u00edn hi\u1ec7u d\u01b0\u01a1ng t\u00ednh. Cu\u1ed1i c\u00f9ng, m\u00e0ng \u0111\u01b0\u1ee3c qu\u00e9t \u0111\u1ec3 ph\u00e1t hi\u1ec7n t\u00edn hi\u1ec7u v\u00e0 x\u00e1c \u0111\u1ecbnh s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a protein c\u1ea7n x\u00e1c \u0111\u1ecbnh.<\/p>\n<p>K\u1ef9 thu\u1eadt Western blot l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch m\u1ea1nh m\u1ebd \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh s\u1ef1 hi\u1ec7n di\u1ec7n v\u00e0 m\u1ee9c \u0111\u1ed9 c\u1ee7a protein c\u1ee5 th\u1ec3 trong m\u1eabu. N\u00f3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i trong nghi\u00ean c\u1ee9u sinh h\u1ecdc, y h\u1ecdc, v\u00e0 c\u00e1c l\u0129nh v\u1ef1c li\u00ean quan \u0111\u1ebfn protein \u0111\u1ec3 nghi\u00ean c\u1ee9u ch\u1ee9c n\u0103ng, t\u01b0\u01a1ng t\u00e1c v\u00e0 bi\u1ec3u hi\u1ec7n protein. Tuy nhi\u00ean, qu\u00e1 tr\u00ecnh Western blot c\u1ea7n \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n c\u1ea9n th\u1eadn v\u00e0 ch\u00ednh x\u00e1c \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o t\u00ednh ch\u00ednh x\u00e1c v\u00e0 \u0111\u1ed9 nh\u1ea1y c\u1ee7a k\u1ebft qu\u1ea3.<\/p>\n<p><strong>4. K\u1ef9 thu\u1eadt Eastern blot\u00a0<\/strong><\/p>\n<p>K\u1ef9 thu\u1eadt Eastern blot l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch v\u00e0 x\u00e1c \u0111\u1ecbnh s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a c\u00e1c ch\u1ea5t nh\u01b0 glycoprotein, glycolipid v\u00e0 c\u00e1c ch\u1ea5t kh\u00e1c c\u00f3 ch\u1ee9a cacbonhydrat trong m\u1ed9t m\u1eabu. \u0110\u00e2y l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p t\u01b0\u01a1ng t\u1ef1 nh\u01b0 Western blot, tuy nhi\u00ean s\u1eed d\u1ee5ng c\u00e1c kh\u00e1ng th\u1ec3 \u0111\u1eb7c hi\u1ec7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n c\u00e1c ch\u1ea5t m\u00f4 h\u00ecnh c\u00f3 ch\u1ee9a cacbonhydrat thay v\u00ec protein.<\/p>\n<p>Qu\u00e1 tr\u00ecnh Eastern blot bao g\u1ed3m c\u00e1c b\u01b0\u1edbc nh\u01b0 sau: \u0111\u1ea7u ti\u00ean, m\u1eabu \u0111\u01b0\u1ee3c t\u00e1ch ra b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p \u0111i\u1ec7n di tr\u00ean gel SDS-PAGE \u0111\u1ec3 ph\u00e2n t\u00e1ch c\u00e1c ch\u1ea5t m\u00f4 h\u00ecnh theo k\u00edch th\u01b0\u1edbc. Sau \u0111\u00f3, c\u00e1c ch\u1ea5t m\u00f4 h\u00ecnh \u0111\u01b0\u1ee3c chuy\u1ec3n t\u1eeb gel l\u00ean m\u00e0ng nitrocellulose ho\u1eb7c PVDF v\u00e0 \u0111\u01b0\u1ee3c l\u00e0m kh\u00f4. M\u00e0ng \u0111\u01b0\u1ee3c x\u1eed l\u00fd b\u1eb1ng c\u00e1c kh\u00e1ng th\u1ec3 \u0111\u1eb7c hi\u1ec7u \u0111\u1ec3 ph\u00e1t hi\u1ec7n s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a c\u00e1c ch\u1ea5t m\u00f4 h\u00ecnh c\u00f3 ch\u1ee9a cacbonhydrat. Th\u00f4ng th\u01b0\u1eddng, kh\u00e1ng th\u1ec3 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c g\u1eafn v\u1edbi m\u1ed9t ph\u1ea7n t\u1eed s\u1eafc t\u1ed1 ho\u1eb7c enzyme \u0111\u1ec3 t\u1ea1o ra t\u00edn hi\u1ec7u d\u01b0\u01a1ng t\u00ednh. Cu\u1ed1i c\u00f9ng, m\u00e0ng \u0111\u01b0\u1ee3c qu\u00e9t \u0111\u1ec3 ph\u00e1t hi\u1ec7n t\u00edn hi\u1ec7u v\u00e0 x\u00e1c \u0111\u1ecbnh s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a c\u00e1c ch\u1ea5t m\u00f4 h\u00ecnh c\u00f3 ch\u1ee9a cacbonhydrat c\u1ea7n x\u00e1c \u0111\u1ecbnh.<\/p>\n<p>K\u1ef9 thu\u1eadt Eastern blot l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p quan tr\u1ecdng \u0111\u1ec3 ph\u00e2n t\u00edch c\u00e1c ch\u1ea5t m\u00f4 h\u00ecnh c\u00f3 ch\u1ee9a cacbonhydrat trong m\u1eabu. N\u00f3 c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 kh\u1ea3o s\u00e1t s\u1ef1 bi\u1ebfn \u0111\u1ed5i trong bi\u1ec3u hi\u1ec7n c\u1ee7a c\u00e1c ch\u1ea5t m\u00f4 h\u00ecnh c\u00f3 ch\u1ee9a cacbonhydrat trong \u0111i\u1ec1u ki\u1ec7n kh\u00e1c nhau ho\u1eb7c t\u00ecm ki\u1ebfm c\u00e1c ch\u1ea5t m\u00f4 h\u00ecnh m\u1edbi. Tuy nhi\u00ean, k\u1ef9 thu\u1eadt n\u00e0y c\u1ea7n \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n c\u1ea9n th\u1eadn v\u00e0 ch\u00ednh x\u00e1c \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o t\u00ednh ch\u00ednh x\u00e1c v\u00e0 \u0111\u1ed9 nh\u1ea1y c\u1ee7a k\u1ebft qu\u1ea3. Ngo\u00e0i ra, k\u1ef9 thu\u1eadt Eastern blot c\u0169ng \u0111\u00f2i h\u1ecfi s\u1ef1 chu\u1ea9n b\u1ecb v\u00e0 x\u1eed l\u00fd m\u1eabu ph\u1ee9c t\u1ea1p h\u01a1n so v\u1edbi Western blot, do \u0111\u00f3 c\u1ea7n ng\u01b0\u1eddi c\u00f3 chuy\u00ean m\u00f4n v\u00e0 kinh nghi\u1ec7m \u0111\u1ec3 th\u1ef1c hi\u1ec7n th\u00e0nh c\u00f4ng.<\/p>\n<p><strong>5. \u0110i\u1ec3m kh\u00e1c bi\u1ec7t ch\u00ednh y\u1ebfu c\u1ee7a 4 ph\u01b0\u01a1ng ph\u00e1p\u00a0<\/strong><\/p>\n<ul>\n<li><strong>Northern blot:<\/strong> ph\u00e2n t\u00edch v\u00e0 x\u00e1c \u0111\u1ecbnh m\u1ee9c \u0111\u1ed9 bi\u1ec3u hi\u1ec7n c\u1ee7a <strong>RNA<\/strong> trong m\u1ed9t m\u1eabu.<\/li>\n<li><strong><a class=\"Markdown_link__9ACHA MarkdownLink_linkifiedLink__KxC9G\">Southern blot<\/a>:<\/strong> ph\u00e2n t\u00edch v\u00e0 x\u00e1c \u0111\u1ecbnh s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a <strong>DNA<\/strong> c\u1ee5 th\u1ec3 trong m\u1ed9t m\u1eabu.<\/li>\n<li><strong>Eastern blot:<\/strong> ph\u00e2n t\u00edch v\u00e0 x\u00e1c \u0111\u1ecbnh s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a <strong>c\u00e1c ch\u1ea5t c\u00f3 ch\u1ee9a cacbonhydrat<\/strong> trong m\u1ed9t m\u1eabu.<\/li>\n<li><strong>Western blot:<\/strong> ph\u00e2n t\u00edch v\u00e0 x\u00e1c \u0111\u1ecbnh m\u1ee9c \u0111\u1ed9 bi\u1ec3u hi\u1ec7n c\u1ee7a <strong>protein<\/strong> trong m\u1ed9t m\u1eabu.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Northern, Southern, Eastern v\u00e0 Western blot l\u00e0 4 k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch ph\u00e2n t\u1eed v\u1edbi nhi\u1ec1u \u1ee9ng d\u1ee5ng nh\u01b0ng d\u1ec5 g\u00e2y nh\u1ea7m l\u1eabn v\u00e0 kh\u00f3 ph\u00e2n bi\u1ec7t. B\u00e0i vi\u1ebft s\u1ebd cung c\u1ea5p nh\u1eefng \u0111i\u1ec3m ch\u00ednh y\u1ebfu v\u00e0 ph\u00e2n bi\u1ec7t 4 ph\u01b0\u01a1ng ph\u00e1p n\u00e0y. 1. K\u1ef9 thu\u1eadt Nothern blot K\u1ef9 thu\u1eadt Northern blot l\u00e0 m\u1ed9t ph\u01b0\u01a1ng [&hellip;]<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[33,14],"tags":[3],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v20.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Ph\u00e2n bi\u1ec7t Northern, Southern, Eastern v\u00e0 Western blot - Vinmec<\/title>\n<meta name=\"description\" content=\"Northern, Southern, Eastern v\u00e0 Western blot l\u00e0 4 k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch ph\u00e2n t\u1eed v\u1edbi nhi\u1ec1u \u1ee9ng d\u1ee5ng nh\u01b0ng d\u1ec5 g\u00e2y nh\u1ea7m l\u1eabn v\u00e0 kh\u00f3 ph\u00e2n bi\u1ec7t.\" \/>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Ph\u00e2n bi\u1ec7t Northern, Southern, Eastern v\u00e0 Western blot - 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