{"id":2236,"date":"2023-04-11T22:07:02","date_gmt":"2023-04-11T15:07:02","guid":{"rendered":"http:\/\/noithathoaphat.in\/ctv\/?p=2236"},"modified":"2023-04-17T16:34:48","modified_gmt":"2023-04-17T09:34:48","slug":"cac-cong-cu-ho-tro-thiet-ke-moi-cho-pcr","status":"publish","type":"post","link":"http:\/\/noithathoaphat.in\/ctv\/index.php\/2023\/04\/11\/cac-cong-cu-ho-tro-thiet-ke-moi-cho-pcr\/","title":{"rendered":"C\u00e1c c\u00f4ng c\u1ee5 h\u1ed7 tr\u1ee3 thi\u1ebft k\u1ebf m\u1ed3i cho PCR"},"content":{"rendered":"<p><strong>Thi\u1ebft k\u1ebf v\u00e0 ki\u1ec3m tra m\u1ed3i cho ph\u1ea3n \u1ee9ng PCR l\u00e0 m\u1ed9t qu\u00e1 tr\u00ecnh ph\u1ee9c t\u1ea1p. Tuy nhi\u00ean, hi\u1ec7n nay c\u00f3 r\u1ea5t nhi\u1ec1u c\u00f4ng c\u1ee5 h\u1ed7 tr\u1ee3 thi\u1ebft k\u1ebf m\u1ed3i cho ph\u1ea3n \u1ee9ng PCR. B\u00e0i vi\u1ebft s\u1ebd gi\u1edbi thi\u1ec7u c\u00e1c c\u00f4ng c\u1ee5 tr\u1ef1c tuy\u1ebfn h\u1ed7 tr\u1ee3 thi\u1ebft k\u1ebf m\u1ed3i, ki\u1ec3m tra m\u1ed3i v\u00e0 c\u00e1c sai l\u1ea7m th\u01b0\u1eddng g\u1eb7p khi thi\u1ebft k\u1ebf.<\/strong><\/p>\n<h4>1. C\u00e1c c\u00f4ng c\u1ee5 tr\u1ef1c tuy\u1ebfn h\u1ed7 tr\u1ee3 thi\u1ebft k\u1ebf m\u1ed3i<\/h4>\n<p>Hi\u1ec7n nay, c\u00f3 r\u1ea5t nhi\u1ec1u c\u00f4ng c\u1ee5 thi\u1ebft k\u1ebf m\u1ed3i tr\u1ef1c tuy\u1ebfn \u0111\u01b0\u1ee3c ph\u00e1t tri\u1ec3n \u0111\u1ec3 h\u1ed7 tr\u1ee3 ng\u01b0\u1eddi d\u00f9ng trong qu\u00e1 tr\u00ecnh thi\u1ebft k\u1ebf m\u1ed3i cho ph\u1ea3n \u1ee9ng PCR. D\u01b0\u1edbi \u0111\u00e2y l\u00e0 m\u1ed9t s\u1ed1 c\u00f4ng c\u1ee5 thi\u1ebft k\u1ebf m\u1ed3i ph\u1ed5 bi\u1ebfn:<\/p>\n<ul>\n<li><strong>Primer3:<\/strong> L\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 thi\u1ebft k\u1ebf m\u1ed3i mi\u1ec5n ph\u00ed v\u00e0 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i. C\u00f4ng c\u1ee5 n\u00e0y cho ph\u00e9p ng\u01b0\u1eddi d\u00f9ng thi\u1ebft k\u1ebf m\u1ed3i cho PCR, sequencing, v\u00e0 c\u00e1c k\u1ef9 thu\u1eadt sinh h\u1ecdc ph\u00e2n t\u1eed c\u1ea7n m\u1ed3i kh\u00e1c.<\/li>\n<li><strong>IDT PrimerQuest:<\/strong> L\u00e0 c\u00f4ng c\u1ee5 thi\u1ebft k\u1ebf m\u1ed3i do Integrated DNA Technologies (IDT) ph\u00e1t tri\u1ec3n. C\u00f4ng c\u1ee5 n\u00e0y cho ph\u00e9p ng\u01b0\u1eddi d\u00f9ng thi\u1ebft k\u1ebf m\u1ed3i cho PCR, qPCR, sequencing, v\u00e0 c\u00e1c \u1ee9ng d\u1ee5ng kh\u00e1c. C\u00f4ng c\u1ee5 n\u00e0y c\u0169ng cung c\u1ea5p th\u00f4ng tin v\u1ec1 t\u00ednh ch\u1ea5t h\u00f3a h\u1ecdc c\u1ee7a m\u1ed3i, nh\u01b0 nhi\u1ec7t \u0111\u1ed9 n\u00f3ng ch\u1ea3y (Tm) v\u00e0 t\u1ef7 l\u1ec7 G\/C.<\/li>\n<li><strong>NCBI Primer-BLAST:<\/strong> L\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 thi\u1ebft k\u1ebf m\u1ed3i mi\u1ec5n ph\u00ed \u0111\u01b0\u1ee3c cung c\u1ea5p b\u1edfi\u00a0<a class=\"Markdown_link__9ACHA MarkdownLink_linkifiedLink__KxC9G\">National Center for Biotechnology Information<\/a> (NCBI). C\u00f4ng c\u1ee5 n\u00e0y cho ph\u00e9p ng\u01b0\u1eddi d\u00f9ng t\u00ecm ki\u1ebfm m\u1ed3i trong c\u01a1 s\u1edf d\u1eef li\u1ec7u\u00a0<a class=\"Markdown_link__9ACHA MarkdownLink_linkifiedLink__KxC9G\">NCBI<\/a>\u00a0v\u00e0 ki\u1ec3m tra s\u1ef1 \u0111\u1eb7c hi\u1ec7u c\u1ee7a m\u1ed3i.<\/li>\n<li><strong>OligoCalc:<\/strong> L\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 t\u00ednh to\u00e1n v\u00e0 thi\u1ebft k\u1ebf m\u1ed3i mi\u1ec5n ph\u00ed. C\u00f4ng c\u1ee5 n\u00e0y cho ph\u00e9p ng\u01b0\u1eddi d\u00f9ng t\u00ednh to\u00e1n nhi\u1ec7t \u0111\u1ed9 n\u00f3ng ch\u1ea3y, t\u1ef7 l\u1ec7 G\/C, \u0111\u1ed9 b\u1ec1n c\u1ee7a m\u1ed3i, v\u00e0 c\u00e1c th\u00f4ng s\u1ed1 kh\u00e1c li\u00ean quan \u0111\u1ebfn m\u1ed3i.<\/li>\n<\/ul>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"size-medium wp-image-2238 aligncenter\" src=\"http:\/\/noithathoaphat.in\/ctv\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-08-at-20.51.55-300x197.png\" alt=\"\" width=\"300\" height=\"197\" srcset=\"http:\/\/noithathoaphat.in\/ctv\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-08-at-20.51.55-300x197.png 300w, http:\/\/noithathoaphat.in\/ctv\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-08-at-20.51.55-768x506.png 768w, http:\/\/noithathoaphat.in\/ctv\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-08-at-20.51.55.png 796w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p>Tuy nhi\u00ean, c\u1ea7n l\u01b0u \u00fd r\u1eb1ng c\u00e1c c\u00f4ng c\u1ee5 thi\u1ebft k\u1ebf m\u1ed3i tr\u1ef1c tuy\u1ebfn ch\u1ec9 cung c\u1ea5p \u0111\u01b0\u1ee3c k\u1ebft qu\u1ea3 t\u01b0\u01a1ng \u0111\u1ed1i v\u00e0 kh\u00f4ng th\u1ec3 thay th\u1ebf cho s\u1ef1 ki\u1ec3m tra v\u00e0 \u0111\u00e1nh gi\u00e1 c\u1ee7a ng\u01b0\u1eddi thi\u1ebft k\u1ebf. Do \u0111\u00f3, vi\u1ec7c ki\u1ec3m tra t\u00ednh \u0111\u1eb7c hi\u1ec7u v\u00e0 \u0111\u1ed9c l\u1eadp c\u1ee7a m\u1ed3i l\u00e0 r\u1ea5t quan tr\u1ecdng \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o hi\u1ec7u qu\u1ea3 v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c c\u1ee7a ph\u1ea3n \u1ee9ng PCR.<\/p>\n<h4>2. C\u00e1c c\u00f4ng c\u1ee5 h\u1ed7 tr\u1ee3 ki\u1ec3m tra \u0111\u1ed9 \u0111\u1eb7c hi\u1ec7u v\u00e0 \u0111\u1ed9c l\u1eadp c\u1ee7a m\u1ed3i<\/h4>\n<p>Ki\u1ec3m tra t\u00ednh \u0111\u1eb7c hi\u1ec7u v\u00e0 \u0111\u1ed9c l\u1eadp c\u1ee7a m\u1ed3i l\u00e0 m\u1ed9t b\u01b0\u1edbc quan tr\u1ecdng trong qu\u00e1 tr\u00ecnh thi\u1ebft k\u1ebf m\u1ed3i cho ph\u1ea3n \u1ee9ng PCR. Sau \u0111\u00e2y l\u00e0 m\u1ed9t s\u1ed1 ph\u01b0\u01a1ng ph\u00e1p ki\u1ec3m tra t\u00ednh \u0111\u1eb7c hi\u1ec7u v\u00e0 \u0111\u1ed9c l\u1eadp c\u1ee7a m\u1ed3i:<\/p>\n<ul>\n<li><strong>BLAST<\/strong>: S\u1eed d\u1ee5ng c\u00f4ng c\u1ee5 BLAST \u0111\u1ec3 t\u00ecm ki\u1ebfm v\u00e0 so s\u00e1nh chu\u1ed7i m\u1ed3i v\u1edbi c\u00e1c chu\u1ed7i trong c\u01a1 s\u1edf d\u1eef li\u1ec7u GenBank. N\u1ebfu m\u1ed3i c\u00f3 \u0111\u1ed9 t\u01b0\u01a1ng \u0111\u1ed3ng cao v\u1edbi c\u00e1c chu\u1ed7i kh\u00e1c ngo\u00e0i v\u00f9ng c\u1ea7n nh\u00e2n b\u1ea3n, th\u00ec m\u1ed3i s\u1ebd kh\u00f4ng \u0111\u1eb7c hi\u1ec7u v\u00e0 c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn k\u1ebft qu\u1ea3 sai.<\/li>\n<li><strong>Gel electrophoresis (\u0111i\u1ec7n di gel)<\/strong>: S\u1eed d\u1ee5ng gel electrophoresis \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh k\u00edch th\u01b0\u1edbc c\u1ee7a s\u1ea3n ph\u1ea9m PCR. N\u1ebfu s\u1ea3n ph\u1ea9m PCR c\u00f3 k\u00edch th\u01b0\u1edbc t\u01b0\u01a1ng \u0111\u1ed3ng v\u1edbi nh\u1eefng s\u1ea3n ph\u1ea9m PCR \u0111\u01b0\u1ee3c t\u1ea1o ra b\u1edfi c\u00e1c m\u1ed3i kh\u00e1c, th\u00ec m\u1ed3i kh\u00f4ng \u0111\u1ed9c l\u1eadp v\u00e0 c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn k\u1ebft qu\u1ea3 sai.<\/li>\n<li><strong><a class=\"Markdown_link__9ACHA MarkdownLink_linkifiedLink__KxC9G\">Melting curve analysis (ph\u00e2n t\u00edch \u0111\u01b0\u1eddng cong c\u1ee7a nhi\u1ec7t \u0111\u1ed9 n\u00f3ng ch\u1ea3y)<\/a>:<\/strong> S\u1eed d\u1ee5ng\u00a0<a class=\"Markdown_link__9ACHA MarkdownLink_linkifiedLink__KxC9G\">melting curve analysis<\/a> \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh t\u00ednh \u0111\u1eb7c hi\u1ec7u c\u1ee7a m\u1ed3i. Melting curve analysis cho ph\u00e9p x\u00e1c \u0111\u1ecbnh Tm c\u1ee7a s\u1ea3n ph\u1ea9m PCR. N\u1ebfu s\u1ea3n ph\u1ea9m PCR c\u00f3 nhi\u1ec7t \u0111\u1ed9 s\u00f4i \u0111\u00fang v\u1edbi m\u1ed3i thi\u1ebft k\u1ebf, th\u00ec m\u1ed3i l\u00e0 \u0111\u1eb7c hi\u1ec7u.<\/li>\n<li><strong><a class=\"Markdown_link__9ACHA MarkdownLink_linkifiedLink__KxC9G\">Specificity testing (ki\u1ec3m tra \u0111\u1eb7c hi\u1ec7u)<\/a>:<\/strong> Ki\u1ec3m tra \u0111\u1eb7c hi\u1ec7u c\u1ee7a m\u1ed3i b\u1eb1ng c\u00e1ch ki\u1ec3m tra cho ph\u1ea3n \u1ee9ng PCR ch\u1ee9a nhi\u1ec1u m\u1eabu kh\u00e1c nhau. N\u1ebfu s\u1ea3n ph\u1ea9m PCR ch\u1ec9 xu\u1ea5t hi\u1ec7n trong ph\u1ea3n \u1ee9ng ch\u1ee9a m\u1eabu c\u1ea7n nh\u00e2n b\u1ea3n, th\u00ec m\u1ed3i l\u00e0 \u0111\u1eb7c hi\u1ec7u.<\/li>\n<li><strong>T\u1ed1i \u01b0u h\u00f3a:<\/strong> T\u1ed1i \u01b0u h\u00f3a \u0111i\u1ec1u ki\u1ec7n PCR \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o r\u1eb1ng s\u1ea3n ph\u1ea9m PCR ch\u1ec9 \u0111\u01b0\u1ee3c nh\u00e2n b\u1ea3n t\u1eeb m\u1eabu c\u1ea7n nh\u00e2n b\u1ea3n. C\u00e1c y\u1ebfu t\u1ed1 c\u1ea7n t\u1ed1i \u01b0u h\u00f3a bao g\u1ed3m n\u1ed3ng \u0111\u1ed9 m\u1ed3i, nhi\u1ec7t \u0111\u1ed9 b\u1eaft c\u1eb7p, v\u00e0 n\u1ed3ng \u0111\u1ed9 Mg2+.<\/li>\n<\/ul>\n<p>T\u1ea5t c\u1ea3 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p tr\u00ean \u0111\u1ec1u c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ki\u1ec3m tra t\u00ednh \u0111\u1eb7c hi\u1ec7u v\u00e0 \u0111\u1ed9c l\u1eadp c\u1ee7a m\u1ed3i. Vi\u1ec7c ki\u1ec3m tra n\u00e0y l\u00e0 r\u1ea5t quan tr\u1ecdng \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o \u0111\u1ed9 ch\u00ednh x\u00e1c c\u1ee7a ph\u1ea3n \u1ee9ng PCR v\u00e0 tr\u00e1nh k\u1ebft qu\u1ea3 sai.<\/p>\n<h4>3. C\u00e1c sai l\u1ea7m hay g\u1eb7p khi thi\u1ebft k\u1ebf m\u1ed3i<\/h4>\n<p>Thi\u1ebft k\u1ebf m\u1ed3i PCR l\u00e0 m\u1ed9t b\u01b0\u1edbc quan tr\u1ecdng trong quy tr\u00ecnh PCR v\u00e0 c\u00f3 m\u1ed9t s\u1ed1 sai l\u1ea7m ph\u1ed5 bi\u1ebfn c\u1ea7n tr\u00e1nh \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o th\u00e0nh c\u00f4ng c\u1ee7a ph\u1ea3n \u1ee9ng. M\u1ed9t s\u1ed1 l\u1ed7i ph\u1ed5 bi\u1ebfn c\u1ea7n tr\u00e1nh khi thi\u1ebft k\u1ebf \u0111o\u1ea1n m\u1ed3i PCR bao g\u1ed3m:<\/p>\n<ul>\n<li><strong>Ch\u1ecdn sai tr\u00ecnh t\u1ef1 \u0111\u00edch:<\/strong> Tr\u00ecnh t\u1ef1 \u0111\u00edch c\u1ea7n \u0111\u01b0\u1ee3c l\u1ef1a ch\u1ecdn c\u1ea9n th\u1eadn \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o r\u1eb1ng n\u00f3 \u0111\u1eb7c tr\u01b0ng cho s\u1ea3n ph\u1ea9m PCR mong mu\u1ed1n. Vi\u1ec7c ch\u1ecdn sai tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn vi\u1ec7c khu\u1ebfch \u0111\u1ea1i c\u00e1c s\u1ea3n ph\u1ea9m ngo\u00e0i \u00fd mu\u1ed1n ho\u1eb7c kh\u00f4ng c\u00f3 s\u1ea3n ph\u1ea9m n\u00e0o c\u1ea3.<\/li>\n<li><strong>Thi\u1ebft k\u1ebf m\u1ed3i c\u00f3 \u0111\u1ed9 d\u00e0i ho\u1eb7c Tm kh\u00f4ng ch\u00ednh x\u00e1c:<\/strong> \u0110\u1ed9 d\u00e0i v\u00e0 Tm c\u1ee7a m\u1ed3i l\u00e0 hai th\u00f4ng s\u1ed1 quan tr\u1ecdng c\u1ea7n \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u h\u00f3a \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o hi\u1ec7u qu\u1ea3 c\u1ee7a ph\u1ea3n \u1ee9ng PCR. \u0110o\u1ea1n m\u1ed3i qu\u00e1 d\u00e0i ho\u1eb7c ng\u1eafn ho\u1eb7c c\u00f3 Tm kh\u00f4ng ch\u00ednh x\u00e1c c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn qu\u00e1 tr\u00ecnh khu\u1ebfch \u0111\u1ea1i kh\u00f4ng hi\u1ec7u qu\u1ea3 ho\u1eb7c s\u1ea3n ph\u1ea9m kh\u00f4ng \u0111\u1eb7c hi\u1ec7u.<\/li>\n<li><strong>Thi\u1ebft k\u1ebf c\u00e1c \u0111o\u1ea1n m\u1ed3i c\u00f3 t\u00ednh t\u1ef1 b\u1ed5 sung ho\u1eb7c b\u1ed5 sung cho nhau:<\/strong> C\u00e1c \u0111o\u1ea1n m\u1ed3i c\u00f3 t\u00ednh t\u1ef1 b\u1ed5 sung ho\u1eb7c b\u1ed5 sung cho nhau c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn s\u1ef1 h\u00ecnh th\u00e0nh khu v\u1ef1c t\u00e1i t\u1ed5 h\u1ee3p, \u0111i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 c\u1ea3n tr\u1edf hi\u1ec7u qu\u1ea3 c\u1ee7a ph\u1ea3n \u1ee9ng PCR.<\/li>\n<li><strong>Kh\u00f4ng x\u00e9t \u0111\u1ebfn s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a \u0111\u1ed9t bi\u1ebfn ho\u1eb7c \u0111a h\u00ecnh:<\/strong> \u0110\u1ed9t bi\u1ebfn ho\u1eb7c \u0111a h\u00ecnh trong tr\u00ecnh t\u1ef1 \u0111\u00edch c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn t\u00ednh \u0111\u1eb7c hi\u1ec7u c\u1ee7a ph\u1ea3n \u1ee9ng PCR. \u0110i\u1ec1u quan tr\u1ecdng l\u00e0 thi\u1ebft k\u1ebf c\u00e1c \u0111o\u1ea1n m\u1ed3i c\u00f3 th\u1ec3 ph\u00e2n bi\u1ec7t gi\u1eefa tr\u00ecnh t\u1ef1 m\u1ee5c ti\u00eau v\u00e0 b\u1ea5t k\u1ef3 \u0111\u1ed9t bi\u1ebfn ho\u1eb7c \u0111a h\u00ecnh ti\u1ec1m \u1ea9n n\u00e0o.<\/li>\n<li><strong>S\u1eed d\u1ee5ng \u0111o\u1ea1n m\u1ed3i c\u00f3 v\u1ecb tr\u00ed g\u1eafn k\u1ebft kh\u00f4ng \u0111\u1eb7c hi\u1ec7u:<\/strong> \u0110o\u1ea1n m\u1ed3i n\u00ean \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf \u0111\u1ec3 tr\u00e1nh c\u00e1c v\u1ecb tr\u00ed g\u1eafn k\u1ebft kh\u00f4ng \u0111\u1eb7c hi\u1ec7u, ch\u1eb3ng h\u1ea1n nh\u01b0 tr\u00ecnh t\u1ef1 l\u1eb7p l\u1ea1i ho\u1eb7c v\u00f9ng c\u00f3 \u0111\u1ed9 ph\u1ee9c t\u1ea1p th\u1ea5p, c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn s\u1ea3n ph\u1ea9m kh\u00f4ng \u0111\u1eb7c hi\u1ec7u.<\/li>\n<li><strong>Kh\u00f4ng t\u1ed1i \u01b0u h\u00f3a c\u00e1c \u0111i\u1ec1u ki\u1ec7n PCR:<\/strong> C\u00e1c \u0111i\u1ec1u ki\u1ec7n PCR nh\u01b0 nhi\u1ec7t \u0111\u1ed9 \u1ee7, th\u1eddi gian k\u00e9o d\u00e0i v\u00e0 n\u1ed3ng \u0111\u1ed9 Mg2+ c\u1ea7n \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u h\u00f3a \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o hi\u1ec7u qu\u1ea3 v\u00e0 t\u00ednh \u0111\u1eb7c hi\u1ec7u c\u1ee7a ph\u1ea3n \u1ee9ng PCR.<\/li>\n<\/ul>\n<p>Nh\u00ecn chung, vi\u1ec7c thi\u1ebft k\u1ebf m\u1ed3i PCR \u0111\u00f2i h\u1ecfi ph\u1ea3i xem x\u00e9t c\u1ea9n th\u1eadn m\u1ed9t s\u1ed1 y\u1ebfu t\u1ed1 \u0111\u1ec3 \u0111\u1ea3m b\u1ea3o s\u1ef1 th\u00e0nh c\u00f4ng c\u1ee7a ph\u1ea3n \u1ee9ng PCR. Tr\u00e1nh nh\u1eefng sai l\u1ea7m ph\u1ed5 bi\u1ebfn n\u00e0y c\u00f3 th\u1ec3 gi\u00fap gi\u1ea3m thi\u1ec3u r\u1ee7i ro PCR th\u1ea5t b\u1ea1i ho\u1eb7c t\u1ea1o ra c\u00e1c s\u1ea3n ph\u1ea9m kh\u00f4ng \u0111\u1eb7c hi\u1ec7u.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Thi\u1ebft k\u1ebf v\u00e0 ki\u1ec3m tra m\u1ed3i cho ph\u1ea3n \u1ee9ng PCR l\u00e0 m\u1ed9t qu\u00e1 tr\u00ecnh ph\u1ee9c t\u1ea1p. Tuy nhi\u00ean, hi\u1ec7n nay c\u00f3 r\u1ea5t nhi\u1ec1u c\u00f4ng c\u1ee5 h\u1ed7 tr\u1ee3 thi\u1ebft k\u1ebf m\u1ed3i cho ph\u1ea3n \u1ee9ng PCR. B\u00e0i vi\u1ebft s\u1ebd gi\u1edbi thi\u1ec7u c\u00e1c c\u00f4ng c\u1ee5 tr\u1ef1c tuy\u1ebfn h\u1ed7 tr\u1ee3 thi\u1ebft k\u1ebf m\u1ed3i, ki\u1ec3m tra m\u1ed3i v\u00e0 c\u00e1c sai [&hellip;]<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[33,14],"tags":[3],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v20.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>C\u00e1c c\u00f4ng c\u1ee5 h\u1ed7 tr\u1ee3 thi\u1ebft k\u1ebf m\u1ed3i cho PCR - Vinmec<\/title>\n<meta name=\"description\" content=\"Thi\u1ebft k\u1ebf v\u00e0 ki\u1ec3m tra m\u1ed3i cho ph\u1ea3n \u1ee9ng PCR l\u00e0 m\u1ed9t qu\u00e1 tr\u00ecnh ph\u1ee9c t\u1ea1p. 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