{"id":1949,"date":"2023-04-08T00:12:45","date_gmt":"2023-04-07T17:12:45","guid":{"rendered":"http:\/\/noithathoaphat.in\/ctv\/?p=1949"},"modified":"2023-04-13T12:55:42","modified_gmt":"2023-04-13T05:55:42","slug":"flow-cytometry-uu-va-nhuoc-diem","status":"publish","type":"post","link":"http:\/\/noithathoaphat.in\/ctv\/index.php\/2023\/04\/08\/flow-cytometry-uu-va-nhuoc-diem\/","title":{"rendered":"K\u1ef9 thu\u1eadt Flow cytometry: \u01b0u v\u00e0 nh\u01b0\u1ee3c \u0111i\u1ec3m"},"content":{"rendered":"<p><strong>Flow cytometry l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch t\u1ebf b\u00e0o c\u00f3 nhi\u1ec1u \u1ee9ng d\u1ee5ng trong nghi\u00ean c\u1ee9u sinh h\u1ecdc v\u00e0 y h\u1ecdc v\u1edbi nh\u1eefng \u01b0u \u0111i\u1ec3m v\u00e0 nh\u01b0\u1ee3c \u0111i\u1ec3m \u0111\u1eb7c th\u00f9. B\u00e0i vi\u1ebft s\u1ebd gi\u1edbi thi\u1ec7u t\u1ed5ng quan v\u1ec1 nguy\u00ean l\u00fd, ph\u00e2n t\u00edch \u1ee9ng d\u1ee5ng v\u00e0 nh\u1eefng h\u1ea1n ch\u1ebf c\u1ee7a k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch t\u1ebf b\u00e0o theo d\u00f2ng ch\u1ea3y.<\/strong><\/p>\n<h4>1. Nguy\u00ean l\u00fd c\u1ee7a k\u1ef9 thu\u1eadt Flow Cytometry<\/h4>\n<p>Flow cytometry (ph\u00e2n t\u00edch t\u1ebf b\u00e0o theo d\u00f2ng ch\u1ea3y) l\u00e0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p ph\u00e2n t\u00edch t\u1ebf b\u00e0o \u0111\u01a1n l\u1ebb d\u1ef1a tr\u00ean s\u1ef1 ph\u00e2n t\u00e1n v\u00e0 ph\u1ea3n x\u1ea1 \u00e1nh s\u00e1ng. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i trong nghi\u00ean c\u1ee9u sinh h\u1ecdc, y h\u1ecdc v\u00e0 c\u00f4ng nghi\u1ec7p \u0111\u1ec3 ph\u00e2n t\u00edch c\u00e1c \u0111\u1eb7c t\u00ednh c\u1ee7a t\u1ebf b\u00e0o, virus v\u00e0 c\u00e1c h\u1ea1t nh\u1ecf kh\u00e1c.<\/p>\n<p>Nguy\u00ean l\u00fd c\u1ee7a flow cytometry l\u00e0 \u0111\u01b0a c\u00e1c t\u1ebf b\u00e0o \u0111\u01a1n l\u1ebb th\u00f4ng qua m\u1ed9t d\u00f2ng ch\u1ea3y \u0111\u1ec1u v\u00e0 \u0111o c\u00e1c th\u00f4ng s\u1ed1 c\u1ee7a t\u1ebf b\u00e0o b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng c\u00e1c d\u1ee5ng c\u1ee5 quang h\u1ecdc v\u00e0 \u0111i\u1ec7n t\u1eed. Khi t\u1ebf b\u00e0o \u0111i qua m\u1ed9t v\u00f9ng \u00e1nh s\u00e1ng, n\u00f3 s\u1ebd g\u1eb7p ph\u1ea3i m\u1ed9t d\u00f2ng ch\u1ea3y m\u1ea1nh, khi\u1ebfn cho t\u1ebf b\u00e0o b\u1ecb ph\u00e2n t\u00e1n v\u00e0 ph\u1ea3n x\u1ea1 \u00e1nh s\u00e1ng, t\u1ea1o ra c\u00e1c t\u00edn hi\u1ec7u quang \u0111i\u1ec7n \u0111\u01b0\u1ee3c ghi nh\u1eadn b\u1edfi c\u00e1c c\u1ea3m bi\u1ebfn. C\u00e1c th\u00f4ng s\u1ed1 c\u1ee7a t\u1ebf b\u00e0o nh\u01b0 k\u00edch th\u01b0\u1edbc, h\u00ecnh d\u1ea1ng, \u0111\u1ed9 s\u00e1ng v\u00e0 t\u00ednh ch\u1ea5t b\u1ec1 m\u1eb7t c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c \u0111o v\u00e0 ph\u00e2n t\u00edch, cho ph\u00e9p x\u00e1c \u0111\u1ecbnh c\u00e1c t\u00ednh ch\u1ea5t c\u1ee7a t\u1ebf b\u00e0o.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter wp-image-1963 size-full\" src=\"http:\/\/noithathoaphat.in\/ctv\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-08-at-00.03.16.png\" alt=\"\" width=\"590\" height=\"608\" srcset=\"http:\/\/noithathoaphat.in\/ctv\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-08-at-00.03.16.png 590w, http:\/\/noithathoaphat.in\/ctv\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-08-at-00.03.16-291x300.png 291w\" sizes=\"(max-width: 590px) 100vw, 590px\" \/><\/p>\n<p>Flow cytometry c\u00f3 nhi\u1ec1u \u1ee9ng d\u1ee5ng trong nghi\u00ean c\u1ee9u sinh h\u1ecdc v\u00e0 y h\u1ecdc, bao g\u1ed3m ph\u00e2n t\u00edch ph\u00e2n b\u1ed1 k\u00edch c\u1ee1 v\u00e0 s\u1ed1 l\u01b0\u1ee3ng c\u00e1c lo\u1ea1i t\u1ebf b\u00e0o, x\u00e1c \u0111\u1ecbnh s\u1ef1 t\u1ed3n t\u1ea1i c\u1ee7a c\u00e1c kh\u00e1ng th\u1ec3 v\u00e0 ph\u00e2n t\u00edch ho\u1ea1t \u0111\u1ed9ng c\u1ee7a c\u00e1c t\u1ebf b\u00e0o, nh\u01b0 s\u1ef1 ph\u00e2n chia v\u00e0 ch\u1ebft \u0111i. Flow cytometry c\u0169ng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e2n t\u00edch c\u00e1c \u0111\u1eb7c t\u00ednh c\u1ee7a virus v\u00e0 h\u1ea1t nh\u1ecf kh\u00e1c, nh\u01b0 k\u00edch th\u01b0\u1edbc v\u00e0 h\u00ecnh d\u1ea1ng, \u0111\u1ec3 gi\u00fap x\u00e1c \u0111\u1ecbnh t\u00ecnh tr\u1ea1ng c\u1ee7a ch\u00fang.<\/p>\n<h4>2. \u1ee8ng d\u1ee5ng c\u1ee7a Flow cytometry trong y h\u1ecdc<\/h4>\n<p>Flow cytometry l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 ph\u00e2n t\u00edch t\u1ebf b\u00e0o m\u1ea1nh m\u1ebd v\u1edbi nhi\u1ec1u \u1ee9ng d\u1ee5ng trong y h\u1ecdc v\u00e0 th\u1ef1c h\u00e0nh l\u00e2m s\u00e0ng. M\u1ed9t trong nh\u1eefng \u1ee9ng d\u1ee5ng ch\u00ednh c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p flow cytometry l\u00e0 ph\u00e2n t\u00edch c\u00e1c t\u1ebf b\u00e0o mi\u1ec5n d\u1ecbch, bao g\u1ed3m t\u1ebf b\u00e0o T, t\u1ebf b\u00e0o B v\u00e0 kh\u00e1ng th\u1ec3. flow cytometry c\u0169ng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong ph\u00e2n t\u00edch huy\u1ebft thanh, bao g\u1ed3m ph\u00e9p \u0111o c\u00e1c ch\u1ea5t sinh h\u00f3a v\u00e0 hi\u1ec7u gi\u00e1 kh\u00e1ng th\u1ec3. C\u00e1c \u1ee9ng d\u1ee5ng n\u00e0y c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ch\u1ea9n \u0111o\u00e1n v\u00e0 theo d\u00f5i nhi\u1ec1u lo\u1ea1i b\u1ec7nh, bao g\u1ed3m ung th\u01b0, b\u1ec7nh tim m\u1ea1ch v\u00e0 r\u1ed1i lo\u1ea1n t\u1ef1 mi\u1ec5n d\u1ecbch.<\/p>\n<p>flow cytometry c\u0169ng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 nghi\u00ean c\u1ee9u c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m c\u1ee7a t\u1ebf b\u00e0o ung th\u01b0, bao g\u1ed3m k\u00edch th\u01b0\u1edbc, h\u00ecnh d\u1ea1ng v\u00e0 bi\u1ec3u hi\u1ec7n c\u1ee7a c\u00e1c marker b\u1ec1 m\u1eb7t. Th\u00f4ng tin n\u00e0y c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh ngu\u1ed3n g\u1ed1c c\u1ee7a ung th\u01b0 v\u00e0 l\u1eadp c\u00e1c k\u1ebf ho\u1ea1ch \u0111i\u1ec1u tr\u1ecb. Ngo\u00e0i ra, t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 c\u00e1c t\u1ebf b\u00e0o g\u1ed1c trong qu\u00e1 tr\u00ecnh ph\u1ee5c h\u1ed3i sau ch\u1ea5n th\u01b0\u01a1ng ho\u1eb7c ph\u1eabu thu\u1eadt.<\/p>\n<p>D\u00f2ng t\u1ebf b\u00e0o h\u1ecdc c\u0169ng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 ch\u1ea5t l\u01b0\u1ee3ng c\u1ee7a c\u00e1c s\u1ea3n ph\u1ea9m t\u1ebf b\u00e0o, bao g\u1ed3m t\u1ebf b\u00e0o g\u1ed1c, t\u1ebf b\u00e0o m\u00e1u v\u00e0 t\u1ebf b\u00e0o mi\u1ec5n d\u1ecbch. \u0110i\u1ec1u n\u00e0y r\u1ea5t quan tr\u1ecdng trong vi\u1ec7c \u0111\u1ea3m b\u1ea3o t\u00ednh an to\u00e0n v\u00e0 hi\u1ec7u qu\u1ea3 c\u1ee7a c\u00e1c li\u1ec7u ph\u00e1p t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong th\u1ef1c h\u00e0nh l\u00e2m s\u00e0ng. Nh\u00ecn chung, flow cytometry l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 c\u00f3 gi\u00e1 tr\u1ecb trong y h\u1ecdc v\u00e0 th\u1ef1c h\u00e0nh l\u00e2m s\u00e0ng cung c\u1ea5p c\u00e1i nh\u00ecn s\u00e2u s\u1eafc v\u1ec1 \u0111\u1eb7c \u0111i\u1ec3m t\u1ebf b\u00e0o v\u00e0 t\u00ecnh tr\u1ea1ng b\u1ec7nh, h\u1ed7 tr\u1ee3 ch\u1ea9n \u0111o\u00e1n, \u0111i\u1ec1u tr\u1ecb v\u00e0 theo d\u00f5i.<\/p>\n<p><strong>3. H\u1ea1n ch\u1ebf c\u1ee7a k\u0129 thu\u1eadt Flow cytometry<\/strong><\/p>\n<p>Trong khi t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 m\u1ea1nh m\u1ebd trong th\u1ef1c h\u00e0nh l\u00e2m s\u00e0ng, c\u00f3 m\u1ed9t s\u1ed1 h\u1ea1n ch\u1ebf \u0111\u1ed1i v\u1edbi vi\u1ec7c s\u1eed d\u1ee5ng n\u00f3. M\u1ed9t s\u1ed1 h\u1ea1n ch\u1ebf c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p t\u1ebf b\u00e0o h\u1ecdc d\u00f2ng ch\u1ea3y bao g\u1ed3m:<\/p>\n<ul>\n<li><strong>Chi ph\u00ed:<\/strong> M\u00e1y \u0111o l\u01b0u l\u01b0\u1ee3ng t\u1ebf b\u00e0o, thu\u1ed1c th\u1eed v\u00e0 b\u1ea3o tr\u00ec c\u00f3 th\u1ec3 t\u1ed1n k\u00e9m, \u0111i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 h\u1ea1n ch\u1ebf kh\u1ea3 n\u0103ng ti\u1ebfp c\u1eadn c\u00f4ng ngh\u1ec7 n\u00e0y \u1edf m\u1ed9t s\u1ed1 c\u01a1 s\u1edf ch\u0103m s\u00f3c s\u1ee9c kh\u1ecfe.<\/li>\n<li><strong>Chuy\u00ean m\u00f4n k\u1ef9 thu\u1eadt:<\/strong> Flow cytometry y\u00eau c\u1ea7u chuy\u00ean m\u00f4n k\u1ef9 thu\u1eadt trong v\u1eadn h\u00e0nh thi\u1ebft b\u1ecb, ph\u00e2n t\u00edch v\u00e0 di\u1ec5n gi\u1ea3i d\u1eef li\u1ec7u. \u0110\u00e2y c\u00f3 th\u1ec3 l\u00e0 m\u1ed9t r\u00e0o c\u1ea3n \u0111\u1ed1i v\u1edbi vi\u1ec7c \u00e1p d\u1ee5ng trong m\u1ed9t s\u1ed1 c\u01a1 s\u1edf ch\u0103m s\u00f3c s\u1ee9c kh\u1ecfe.<\/li>\n<li><strong>Chu\u1ea9n b\u1ecb m\u1eabu:<\/strong> Flow cytometry y\u00eau c\u1ea7u chu\u1ea9n b\u1ecb m\u1eabu c\u1ea9n th\u1eadn, c\u00f3 th\u1ec3 t\u1ed1n nhi\u1ec1u th\u1eddi gian v\u00e0 c\u00f4ng s\u1ee9c. \u0110i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 h\u1ea1n ch\u1ebf s\u1ed1 l\u01b0\u1ee3ng m\u1eabu v\u00e0 c\u00f3 th\u1ec3 kh\u00f4ng kh\u1ea3 thi \u0111\u1ed1i v\u1edbi m\u1ed9t s\u1ed1 \u1ee9ng d\u1ee5ng l\u00e2m s\u00e0ng.<\/li>\n<li><strong>T\u00ednh kh\u00f4ng \u0111\u1ed3ng nh\u1ea5t c\u1ee7a t\u1ebf b\u00e0o:<\/strong> Flow cytometry c\u00f3 th\u1ec3 b\u1ecb gi\u1edbi h\u1ea1n b\u1edfi t\u00ednh kh\u00f4ng \u0111\u1ed3ng nh\u1ea5t c\u1ee7a t\u1ebf b\u00e0o, trong \u0111\u00f3 c\u00e1c t\u1ebf b\u00e0o c\u00f3 marker b\u1ec1 m\u1eb7t t\u01b0\u01a1ng t\u1ef1 nhau c\u00f3 th\u1ec3 c\u00f3 c\u00e1c ch\u1ee9c n\u0103ng ho\u1eb7c ph\u1ea3n \u1ee9ng kh\u00e1c nhau \u0111\u1ed1i v\u1edbi vi\u1ec7c \u0111i\u1ec1u tr\u1ecb. \u0110i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 g\u00e2y kh\u00f3 kh\u0103n cho vi\u1ec7c ch\u1ea9n \u0111o\u00e1n ch\u00ednh x\u00e1c ho\u1eb7c theo d\u00f5i m\u1ed9t s\u1ed1 b\u1ec7nh.<\/li>\n<li>Th\u00f4ng tin h\u1ea1n ch\u1ebf: Flow cytometrycung c\u1ea5p th\u00f4ng tin v\u1ec1 c\u00e1c \u0111\u1eb7c \u0111i\u1ec3m c\u1ee7a t\u1ebf b\u00e0o nh\u01b0ng kh\u00f4ng cung c\u1ea5p th\u00f4ng tin v\u1ec1 ch\u1ee9c n\u0103ng c\u1ee7a t\u1ebf b\u00e0o ho\u1eb7c bi\u1ec3u hi\u1ec7n gen c\u1ee7a ch\u00fang. \u0110i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 h\u1ea1n ch\u1ebf \u1ee9ng d\u1ee5ng c\u1ee7a n\u00f3 trong m\u1ed9t s\u1ed1 \u1ee9ng d\u1ee5ng l\u00e2m s\u00e0ng.<\/li>\n<\/ul>\n<p>Ngay c\u1ea3 khi c\u00f3 nh\u1eefng h\u1ea1n ch\u1ebf tr\u00ean, ph\u01b0\u01a1ng ph\u00e1p flow cytometry v\u1eabn l\u00e0 m\u1ed9t c\u00f4ng c\u1ee5 r\u1ea5t c\u00f3 gi\u00e1 tr\u1ecb trong th\u1ef1c h\u00e0nh l\u00e2m s\u00e0ng v\u00e0 c\u00e1c nh\u00e0 nghi\u00ean c\u1ee9u \u0111ang t\u1eebng ng\u00e0y gi\u1ea3i quy\u1ebft c\u00e1c h\u1ea1n ch\u1ebf c\u1ee7a k\u1ef9 thu\u1eadt mang nhi\u1ec1u ti\u1ec1m n\u0103ng n\u00e0y.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Flow cytometry l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch t\u1ebf b\u00e0o c\u00f3 nhi\u1ec1u \u1ee9ng d\u1ee5ng trong nghi\u00ean c\u1ee9u sinh h\u1ecdc v\u00e0 y h\u1ecdc v\u1edbi nh\u1eefng \u01b0u \u0111i\u1ec3m v\u00e0 nh\u01b0\u1ee3c \u0111i\u1ec3m \u0111\u1eb7c th\u00f9. B\u00e0i vi\u1ebft s\u1ebd gi\u1edbi thi\u1ec7u t\u1ed5ng quan v\u1ec1 nguy\u00ean l\u00fd, ph\u00e2n t\u00edch \u1ee9ng d\u1ee5ng v\u00e0 nh\u1eefng h\u1ea1n ch\u1ebf c\u1ee7a k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch t\u1ebf [&hellip;]<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[33,14],"tags":[3],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v20.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>K\u1ef9 thu\u1eadt Flow cytometry: \u01b0u v\u00e0 nh\u01b0\u1ee3c \u0111i\u1ec3m - Vinmec<\/title>\n<meta name=\"description\" content=\"Flow cytometry l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt ph\u00e2n t\u00edch t\u1ebf b\u00e0o c\u00f3 nhi\u1ec1u \u1ee9ng d\u1ee5ng trong nghi\u00ean c\u1ee9u sinh h\u1ecdc v\u00e0 y h\u1ecdc v\u1edbi nh\u1eefng \u01b0u \u0111i\u1ec3m v\u00e0 nh\u01b0\u1ee3c \u0111i\u1ec3m \u0111\u1eb7c th\u00f9.\" \/>\n<meta name=\"robots\" content=\"noindex, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"K\u1ef9 thu\u1eadt Flow cytometry: \u01b0u v\u00e0 nh\u01b0\u1ee3c \u0111i\u1ec3m - 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